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Clin. Vaccine Immunol. doi:10.1128/CVI.00363-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The usefulness of four different Echinococcus granulosus recombinant antigens for the serodiagnosis and follow-up of hydatid patients

IRNASA, CSIC. Cordel de Merinas, 50, 37008 Salamanca, Spain; Laboratorio de Parasitología, CIETUS, Facultad de Farmacia, Universidad de Salamanca. Campo Charro, s/n, 37007 Salamanca, Spain; Departamento de Estadística, Facultad de Biología, Universidad de Salamanca. Campo Charro, s/n, 37007 Salamanca, Spain; Hospital Universitario de Valladolid. Ramón y Cajal, 3, 47003 Valladolid, Spain

^* To whom correspondence should be addressed. Email: marsiles{at}usal.es.

	Abstract

Four different recombinant antigens derived from Echinococcus granulosus, designated B1t, B2t, E14t and C317, were tested with ELISA for the detection of specific IgG in unilocular hydatid disease (UHD) patients. The results were compared with those obtained with hydatid fluid and were subjected to receiver operator characteristic (ROC) analysis. The diagnostic performance of the above proteins was defined in respect to their specificity, sensitivity, predictive values (PV), the influence of cyst location, and usefulness in the follow-up and detection of surgically cured UHD patients. The best diagnostic approach was obtained with anti-B2t IgG ELISA, with 91.2% sensitivity, 93% specificity and high positive and negative PV (89.4 and 94.2, respectively). In addition, this diagnostic tool proved to be useful for the follow-up of surgically-treated UHD patients. Anti-B2t IgG ELISA may find application in the serodiagnosis of UHD in clinical laboratories.