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Clin. Vaccine Immunol. doi:10.1128/CVI.00254-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A Neutralizing Antibody to the A chain of Abrin: In vitro and in vivo protective effect on abrin toxicity

Department of Biochemistry, Indian Institute of Science, Bangalore- 560012, India

^* To whom correspondence should be addressed. Email: anjali{at}biochem.iisc.ernet.in.

	Abstract

Plant ribosome inactivating proteins are RNA-N glycosidases that inhibit protein synthesis in cells. Abrin, a type-II RIP is an AB type toxin, which is one of the most lethal toxins known. The B chain facilitates entry of the molecule in the cell, whereas the A chain exerts its toxic effect. We have generated hybridoma secreting antibodies of IgG class specific to the recombinant A chain of abrin. One monoclonal antibody, namely D6F10 rescued cells from abrin toxicity. Importantly, the antibody also protected mice from lethal doses of the toxin. The neutralizing effect of the antibody was shown to be due to interference with abrin attachment to the cell surface.