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Clin. Vaccine Immunol. doi:10.1128/CVI.00225-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Measuring IgG-antibodies to tetanus toxin, diphtheria toxin and pertussis toxin with single antigen ELISAs and with a bead based multiplex assay

Institut Virion\Serion GmbH, Würzburg, Germany; Institut für Infektiologie Krefeld GmbH, Krefeld, Germany; Helios Klinikum Krefeld, Krefeld, Germany

^* To whom correspondence should be addressed. Email: wvk{at}klinikum-krefeld.de.

	Abstract

Background: Bead based assay systems offer the possibility of measuring several specific antibodies in one sample simultaneously. This study evaluated a vaccine panel of the multianalyte system that measures antibodies to tetanus toxin, diphtheria toxin, and pertussis toxin (PT) from Bordetella pertussis.

Methods: The antibody concentration of human IgG to pertussis toxin, tetanus toxin and diphtheria toxin was measured in 123 serum pairs (total of 246 sera) from a vaccine study. The multianalyte bead assay was compared to a standardized in-house IgG-anti-PT ELISA of the German reference laboratory for Bordetellae as well as to various commercially available ELISAs for anti-PT IgG, anti-tetanus IgG and anti-diphtheria IgG.

Results: The results of the multiplex assay regarding the antibodies against diphtheria toxin compared favorably with a regression coefficient of 0,938 for values obtained with an ELISA from the same manufacturer used as a reference. Similarly, antibodies to tetanus toxin showed a correlation of 0.910 between the reference ELISA and the multianalyte assay. A correlation coefficient of 0.905 was found, when an "in-house" IgG-anti-PT and the multiplex assay were compared. When compared to single ELISA systems from two other manufacturers, the multiplex assay performed similarly well or better.

Conclusion: The multianalyte assay system was a robust system with fast and accurate result analyzing three parameters simultaneously in one sample. The system was well suited to quantitatively determine relevant vaccine induced antibodies, when compared to in-house and commercially available single antigen ELISA systems.