Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP10

doi:10.1128/CDLI.7.2.155-160.2000

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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 155-160, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP10

Laurens A. H. van Pinxteren,¹ Pernille Ravn,¹ Else Marie Agger,¹ John Pollock,² and Peter Andersen¹^,^*

Department of TB-Immunology, Statens Serum Institut, Copenhagen, Denmark,¹ and Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, United Kingdom²

Received 30 July 1999/Returned for modification 3 November 1999/Accepted 11 November 1999

Tests based on tuberculin purified protein derivative (PPD) cannot distinguish between tuberculosis infection, Mycobacteriumbovis BCG vaccination, or exposure to environmental mycobacteria.The present study investigated the diagnostic potential of twoMycobacterium tuberculosis-specific antigens (ESAT-6 and CFP10)in experimental animals as well as during natural infection inhumans and cattle. Both antigens were frequently recognized invivo and in vitro based on the induction of delayed-type hypersensitivityresponses and the ability to induce gamma interferon productionby lymphocytes, respectively. The combination of ESAT-6 and CFP10was found to be highly sensitive and specific for both in vivoand in vitro diagnosis. In humans, the combination had a highsensitivity (73%) and a much higher specificity (93%) than PPD(7%).

^* Corresponding author. Mailing address: Statens Serum Institut, Department of TB-Immunology, Artillerivej 5, 2300 CopenhagenS, Denmark. Phone: 45-32683480. Fax: 45-32683035. E-mail: pa{at}ssi.dk.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 155-160, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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