
Cellular and Humoral Immune Responses to Alphavirus Replicon Vaccines Expressing Cytomegalovirus pp65, IE1, and gB Proteins
Clin. Vaccine Immunol.
Reap et al.
14: 748
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Methods for purification of cytomegalovirus (CMV) proteins, the CMV neutralization assay, and the gamma interferon (IFN-γ) enzyme-linked immunospot (ELISPOT), and figures and tables as follows: Figure S1, showing correlation of results of spectrophotometric and microscopic assays for titration of CMV (A) and neutralization of CMV by serum from BALB/c mice before or after immunization with virus-like replicon particles (VRP) expressing gB or TrgB (B); Fig. S2 and Table S1, showing IFN-γ ELISPOT responses to individual peptides and peptide pools, respectively, following immunization with VRP expressing CMV pp65; and Table S2, showing IFN-γ ELISPOT responses to individual peptides following immunization with VRP expressing CMV IE1.
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