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Clinical and Vaccine Immunology, January 2006, p. 156-159, Vol. 13, No. 1
1071-412X/06/$08.00+0 doi:10.1128/CVI.13.1.156-159.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Department of Anesthesia, Bamberg Hospital and University Hospital Aachen, Bamberg, Germany,1 Laboratory of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands,2 Department of Clinical Chemistry, DRK-Kliniken, Neuwied, Germany3
Received 20 June 2005/ Returned for modification 29 July 2005/ Accepted 25 August 2005
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MCP-1 binds to the G-protein-coupled receptor CC chemokine receptor 2 (CCR2) (2) expressed on the cell surface of monocytes. CCR2 is not a static entity but is subjected to dynamic regulation. TNF-
induced a decrease of the monocyte surface expression of CCR2 (12). Sica et al. (10) reported that lipopolysaccharide (LPS), an outer membrane component of gram-negative bacteria, reduced monocyte CCR2 mRNA levels in vitro by decreasing the mRNA half-life (10). Knowledge of CCR2 expression on monocytes in vivo is limited. Therefore, in the present study, we sought to determine time-dependent alterations in monocyte CCR2 expression after intravenous administration of LPS to healthy humans.
Experimental human endotoxemia. After approval by the local ethics committee and written informed consent, eight healthy men (ages 18 to 35 years) were admitted to the clinical research unit of the Academic Medical Center, University of Amsterdam. The volunteers were in good health as documented by medical history, physical examination, hematologic and biochemical laboratory measurements, electrocardiography, and chest radiography. Exclusion criteria were the use of any medication, smoking, and febrile illness within 2 weeks prior to the start of the study. Four nanograms of LPS per kilogram of body weight (Escherichia coli, lot G; U.S. Pharmacopeia, Rockville, MD) was injected intravenously. Venous blood samples for flow cytometry were taken prior to and 1, 2, 4, 6, and 24 h after LPS injection. These samples were processed immediately. Citrate-anticoagulated blood samples were used for determination of MCP-1 and MCP-3 plasma concentrations. These samples were taken prior to LPS injection and 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, and 24 h thereafter. Samples were centrifuged, and the plasma was stored at –20°C.
Flow cytometry. Heparinized whole blood was used for flow cytometric analysis. After erythrocytes were lysed by incubation with bicarbonate-buffered ammonium chloride solution (pH 7.4), leukocytes were recovered by centrifugation at 400 x g for 10 min. Cells were resuspended in phosphate-buffered saline (containing 100 mM EDTA, 0.1% sodium azide, and 5% bovine serum albumin) and washed twice. Staining was done by incubation for 60 min with allophycocyanin-labeled anti-human CD14 antibodies (BD Diagnostic Systems, Alphen aan den Rijn, The Netherlands) and phycoerythrin-labeled anti-human CCR2 antibodies (immunoglobulin G2a subtype; R&D Systems, Minneapolis, MN). Monocytes were identified as CD14-positive cells within the monocyte gate. Nonspecific staining was assessed by incubation with phycoerythrin-conjugated mouse immunoglobulin G2a (R&D Systems). Cells were then washed twice in ice-cold phosphate-buffered saline and resuspended for flow cytometric analysis (Calibur; Becton-Dickinson Immunocytometry Systems, San Jose, CA). Mean fluorescence intensity (MFI) was calculated as the difference between MFIs of specifically and nonspecifically stained cells. Data are given as the percentage of baseline MFI values.
MCP-1, MCP-3, and TNF- assays.
MCP-1, MCP-3, and TNF- plasma concentrations were measured in duplicate by commercially available enzyme-linked immunosorbent assays (R&D Systems). Detection limits of the assays were 5 pg/ml.
In vitro studies. Whole blood of five healthy volunteers was collected in vials containing heparin. Ten milliliters of whole blood was diluted 1:2 with RPMI 1640 medium (BioWhittaker, Walkersville, MD). LPS (Escherichia coli O55:B5; Difco, Detroit, MI) was added in final concentrations of 100, 10, and 1 ng/ml and 100, 10, 1, 0.1, 0.01, and 0 pg/ml LPS. After 24 h, CCR2 expression was measured as detailed above. CCR2 expression is given as a percentage of the control MFI value (LPS concentration, 0 pg/ml).
Statistics. All values are given as means ± standard errors (SE). Data were analyzed by one-way analysis of variance (CCR2 surface expression) or Kruskal-Wallis test (chemokine plasma concentrations). Statistical significance was assumed if the P value was <0.05.
Human endotoxemia.
LPS administration elicited transient influenza-like symptoms associated with a febrile response peaking after 4 h (38.4 ± 0.38°C). LPS injection led to a decrease in the number of circulating monocytes. Monocyte counts at the time of fluorescence-activated cell sorter analysis were as follows (109 cells/liter): 0.54 ± 0.04 (0 h), 0.01 ± 0.00 (1 h), 0.13 ± 0.06 (4 h), 0.27 ± 0.09 (6 h), and 0.79 ± 0.06 (24 h). LPS injection induced a significant decrease in CCR2 monocyte expression (Fig. 1) (P < 0.01). The most pronounced difference was observed 4 h after injection, when a minimum of 32% of baseline values was reached. Six hours after injection, CCR2 expression had recovered to 47%. A return to baseline values was observed after 24 h. MCP-1 plasma concentrations (picograms per milliliter) are given in Fig. 2. MCP-1 levels were not detectable before LPS injection in any of the volunteers. After LPS administration, MCP-1 plasma levels increased significantly, peaking 4 h after injection. At 24 h, plasma levels were again below the detection limit. TNF- concentrations (Fig. 2) reached a maximum 2 h after LPS administration (P < 0.01). The values were back to baseline values 6 h after LPS was injected. MCP-3 plasma concentrations did not change in response to LPS and were undetectable in all subjects (data not shown).
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CCR2 is crucial for monocyte migration into inflamed tissues and infection with certain pathogens. Mice with a targeted disruption of CCR2 failed to recruit monocytes in a peritonitis model (5). In addition, animals lacking a functional CCR2 displayed a reduced delayed-type hypersensitivity reaction and granuloma formation (1). CCR2 knockout mice were unable to clear infections with the intracellular pathogen Listeria monocytogenes (5). They were also more susceptible to infections with Leishmania major (9) and Cryptococcus neoformans (11). In vivo regulation of CCR2 surface density could be associated with altered susceptibility to infectious agents.
LPS triggers synthesis and release of a variety of cytokines and chemokines including TNF- and MCP-1. Xu and colleagues (14) found that concentrations of 100 to 500 ng/ml MCP-1 were required to down-regulate CCR2 surface expression. These MCP-1 concentrations are by far higher than the peak MCP-1 plasma levels of 14 ng/ml observed during our in vivo study (14).
In our study, TNF- peaked 2 h after LPS administration, i.e., 2 h before the minimum CCR2 expression was observed. Weber et al. found a decrease of monocyte CCR2 to approximately 65% of the control value after the addition of 100 U/ml (12), i.e., 11.4 ng/ml (13). The peak TNF- concentration in our study was only 2.1 ng/ml.
Based on the reported in vitro effects of MCP-1 and TNF-, we may speculate that the in vivo plasma concentrations of both molecules after LPS challenge are too low to induce the CCR2 down-regulation in our study.
By contrast, our in vitro data on LPS demonstrated that LPS is a very strong modulator of CCR2 expression.
Taking all these data together, one may speculate that LPS itself is most likely to account for the down-regulation of CCR2 during human endotoxemia.
Twenty-four hours after LPS injection, CCR2 expression returned to the baseline values. Another group had previously defined the fate of the receptor molecules: upon LPS stimulation, CCR2 is internalized and stored in vesicles (14). A rapid recycling of CCR2 molecules from these vesicles to the cell surface could explain the complete recovery of antibody binding 24 h after LPS injection that was observed in our study.
LPS-induced receptor down-regulation is not limited to CCR2. Previous studies reported a decrease of CCR5 as well as of CXC receptors CXCR2 and CXCR4 surface expression (3, 4). No effect on CXCR1 was observed (4). Similar to our study of CCR2, those reports found a transient decrease in receptor density.
CCR2 plays a major role in infection, as suggested by studies of animals with a targeted disruption of this molecule (5). Studies of the regulation of MCP-1/CCR2 could provide further insight into the mechanisms underlying these entities. From the results of our study, we conclude that LPS in vivo induced a transient increase of MCP-1 and a concomitant decrease of CCR2 density on human monocytes. The peak of the MCP-1 increase coincided with the minimal CCR2 expression. A down-regulation of CCR2 counteracts increases of MCP-1, thereby limiting potentially deleterious effects of high concentrations of this chemokine.
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ACKNOWLEDGMENTS |
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) and TNF-
). Mean (±SE) levels of MCP-1 after lipopolysaccharide administration (4 ng/kg) to healthy human volunteers are shown. The P value indicates significance for changes over time.