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CVI Accepts, published online ahead of print on 30 April 2008
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CVI.00424-07v1
15/6/1031    most recent
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Clin. Vaccine Immunol. doi:10.1128/CVI.00424-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

EVALUATION OF SEVEN TESTS FOR DIAGNOSIS OF HUMAN BRUCELLOSIS IN AN ENDEMIC AREA

Mª Concepción Gómez*, José A. Nieto, Carmen Rosa, Paloma Geijo, Mª Ángeles Escribano, Amparo Muñoz, and Clara López

Microbiology Laboratory Hospital "Severo Ochoa" (Leganés) Madrid; Department of Internal Medicine, Microbiology Laboratory Hospital "Virgen de la Luz" Cuenca, España

* To whom correspondence should be addressed. Email: mcongocri{at}hotmail.com.


  Abstract

The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated rose Bengal test, Microagglutination (MAT) test, microtiter adapted Coombs test and immunocapture-agglutination test (Brucellacapt) were positive in all sera from patients with acute brucellosis. The IgG, IgM and IgA commercial enzyme immunoassay (ELISA) tests failed to show specific antibodies in three, ten and one patients respectively. The sensitivity of ELISA is not higher than that of conventional tests.




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
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Copyright © 2008 by the American Society for Microbiology. All rights reserved.