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Clinical and Diagnostic Laboratory Immunology, September 2002, p. 1137-1141, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.1137-1141.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Diagnostic Techniques To Detect Cryptic Leishmaniasis in Dogs

Laura Iniesta,1 Salceda Fernández-Barredo,2 Béatrice Bulle,3 M. Teresa Gómez,2 Renaud Piarroux,3 Montserrat Gállego,1 José M. Alunda,2 and Montserrat Portús1*

Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona,1 Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain,2 Laboratoire Parasitologie-Mycologie, Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Besançon, France3

Received 26 November 2001/ Returned for modification 7 March 2002/ Accepted 17 May 2002

This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninfected and infected asymptomatic dogs and that culture and PCR are more reliable diagnostic tools.

* Corresponding author. Mailing address: Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal s.n., E-08028 Barcelona, Spain. Phone: 34 93 402 45 00. Fax: 34 93 402 45 04. E-mail: mportus{at}farmacia.far.ub.es.

Clinical and Diagnostic Laboratory Immunology, September 2002, p. 1137-1141, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.1137-1141.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
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Copyright © 2002 by the American Society for Microbiology. All rights reserved.