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Clinical and Diagnostic Laboratory Immunology, September 2002, p. 1025-1031, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.1025-1031.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Serodiagnosis of Mice Minute Virus and Mouse Parvovirus Infections in Mice by Enzyme-Linked Immunosorbent Assay with Baculovirus-Expressed Recombinant VP2 Proteins

Robert S. Livingston,1* David G. Besselsen,2 Earl K. Steffen,1 Cynthia L. Besch-Williford,1 Craig L. Franklin,1 and Lela K. Riley1

Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211,1 University Animal Care, University of Arizona, Tucson, Arizona 85721-01012

Received 7 March 2002/ Returned for modification 6 May 2002/ Accepted 6 May 2002

Mice minute virus (MMV) and mouse parvovirus (MPV) type 1 are the two parvoviruses known to naturally infect laboratory mice and are among the most prevalent infectious agents found in contemporary laboratory mouse colonies. Serologic assays are commonly used to diagnose MMV and MPV infections in laboratory mice; however, highly accurate, high-throughput serologic assays for the detection of MMV- and MPV-infected mice are needed. To this end, the major capsid viral protein (VP2) genes of MMV and MPV were cloned and MMV recombinant VP2 (rVP2) and MPV rVP2 proteins were expressed by using a baculovirus system. MMV rVP2 and MPV rVP2 spontaneously formed virus-like particles that were morphologically similar to empty parvovirus capsids. These proteins were used as antigens in enzyme-linked immunosorbent assays (ELISAs) to detect anti-MMV or anti-MPV antibodies in the sera of infected mice. Sera from mice experimentally infected with MMV (n = 43) or MPV (n = 35) and sera from uninfected mice (n = 30) were used to evaluate the ELISAs. The MMV ELISA was 100% sensitive and 100% specific in detecting MMV-infected mice, and the MPV ELISA was 100% sensitive and 98.6% specific in detecting MPV-infected mice. Both assays outperformed a parvovirus ELISA that uses a recombinant nonstructural protein (NS1) of MMV as antigen. The MMV rVP2 and MPV rVP2 proteins provide a ready source of easily produced antigen, and the ELISAs developed provide highly accurate, high-throughput assays for the serodiagnosis of MMV and MPV infections in laboratory mice.

* Corresponding author. Mailing address: E115A Veterinary Medicine Building, Department of Veterinary Pathobiology, College of Veterinary Medicine, 1600 East Rollins Rd., Columbia, MO 65211. Phone: (573) 884-3126. Fax: (573) 884-7521. E-mail: LivingstonR{at}missouri.edu.

Clinical and Diagnostic Laboratory Immunology, September 2002, p. 1025-1031, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.1025-1031.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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