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Clinical and Diagnostic Laboratory Immunology, March 2002, p. 499-502, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.499-502.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry

Lynn M. Herrmann,1* Timothy V. Baszler,2 Donald P. Knowles,1,2 and William P. Cheevers2

USDA Agricultural Research Service ADRU,1 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 991642

Received 3 October 2001/ Returned for modification 28 November 2001/ Accepted 18 December 2001

Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% ± 0.28% of 3 x 106 DRLN cells, but were not detected in 3 x 106 PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 x 106 PBLs.

* Corresponding author. Mailing address: USDA/ARS/ADRU, 3003 ADBF, Pullman, WA 99164. Phone: (509) 335-6068. Fax: (509) 335-8328. E-mail: lherrman{at}vetmed.wsu.edu.

Clinical and Diagnostic Laboratory Immunology, March 2002, p. 499-502, Vol. 9, No. 2
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.2.499-502.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.