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Sensitive Microplate Assay for Detection of Bactericidal Antibodies to Vibrio cholerae O139

Department of Molecular Biosciences, University of Adelaide, Adelaide, South Australia 5005, Australia,¹ Department of Medical Microbiology and Immunology, Göteborg University, Göteborg, Sweden,² National Institute of Hygiene and Epidemiology, Hanoi, Vietnam,³ International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh⁴

Received 19 October 2001/ Accepted 26 November 2001

A microplate assay for the detection of bactericidal antibodies to Vibrio cholerae O139 is described. The assay is sensitive, highly reproducible, specific, and convenient to perform. It has been used to demonstrate the induction of serum bactericidal antibodies in Vietnamese recipients of an oral, inactivated, bivalent O1/O139 vaccine, as well as in Bangladeshi patients with O139 disease. In both study groups there was a significant inverse correlation between the preexposure level of antibodies in serum and the magnitude of the subsequent bactericidal response. Although infection generated stronger responses than vaccination, the proportion of responders was similar among individuals with low background titers.