CVI
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vaughan, G.
Right arrow Articles by Escobar-Gutiérrez, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vaughan, G.
Right arrow Articles by Escobar-Gutiérrez, A.
Clinical and Diagnostic Laboratory Immunology, January 2002, p. 198-200, Vol. 9, No. 1
1071-412X/01/$04.00+0     DOI: 10.1128/CDLI.9.1.198-200.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dengue Virus Replicative Intermediate RNA Detection by Reverse Transcription-PCR

Gilberto Vaughan,1 Hiram Olivera,1 Leopoldo Santos-Argumedo,2 Abraham Landa,3 Baltasar Briseño,1 and Alejandro Escobar-Gutiérrez1*

Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, México DF 11340,1 Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México DF 07000,2 Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF 04510, México3

Received 27 April 2001/ Returned for modification 15 August 2001/ Accepted 29 October 2001

Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.

* Corresponding author. Mailing address: Departamento de Investigaciones Inmunológicas, Instituto de Diagnóstico y Referencia Epidemiológicos, Carpio 470, Col. Santo Tomas, México DF 11340, México. Phone: (525) 341-1833. Fax: (525) 341-3264. E-mail: aescobaa{at}conacyt.mx.

Clinical and Diagnostic Laboratory Immunology, January 2002, p. 198-200, Vol. 9, No. 1
1071-412X/01/$04.00+0     DOI: 10.1128/CDLI.9.1.198-200.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 2002 by the American Society for Microbiology. All rights reserved.