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Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1267-1270, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1267-1270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Recognition of Multiple Classes of Hepatitis C Antibodies Increases Detection Sensitivity in Oral Fluid

Jonathan F. Zmuda,* Barbara Wagoneer, Lance Liotta, and Gordon Whiteley

Immunomatrix Inc., Gaithersburg, Maryland

Received 11 May 2001/Returned for modification 12 July 2001/Accepted 26 July 2001

Paired serum-oral fluid samples from 127 hepatitis C virus (HCV)-positive and 31 HCV-negative patients were tested for the presence of anti-HCV using the Ortho HCV 3.0 ELISA. Using the immunoglobulin G (IgG)-specific detection antibody provided with the HCV 3.0 ELISA we attained 100% sensitivity and specificity with serum samples; however, sensitivity in oral fluid samples was only 81%. By modifying the HCV 3.0 ELISA to utilize a secondary antibody cocktail that recognizes not only IgG but IgA and IgM as well, we attained 100% specificity and sensitivity with oral fluid samples.

* Corresponding author. Mailing address: Immunomatrix Inc., Gaithersburg, MD 20878. Phone: (301) 208-8000. Fax: (301) 208-9731. E-mail: JonZmuda{at}clark.net.

Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1267-1270, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1267-1270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.