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Clinical and Diagnostic Laboratory Immunology, September 2001, p. 1024-1027, Vol. 8, No. 5
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.5.1024-1027.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Excretory-Secretory Antigens of Trypanosoma cruzi Are Potentially Useful for Serodiagnosis of Chronic Chagas' Disease

Mineo Nakazawa,1 Daniela S. Rosa,1 Valéria R. A. Pereira,1 Milena O. Moura,1 Veridiana C. Furtado,1 Wayner V. Souza,1 Maria Das Neves D. S. Barros,2 Frederico G. C. Abath,1 and Yara M. Gomes1,*

Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ,1 and Ambulatorio de Doença de Chagas, Hospital Universitário Oswaldo Cruz/UPE,2 Recife-PE, Brazil

Received 11 December 2000/Returned for modification 6 March 2001/Accepted 8 June 2001

The reactivities of sera from chronic chagasic patients against the trypomastigote excreted-secreted antigens (TESA) of Trypanosoma cruzi strains with different biodemes were analyzed by TESA-blot and TESA-enzyme-linked immunosorbent assay (ELISA). Although both tests presented high sensitivity and specificity, TESA-ELISA is more appropriate for screening a larger number of samples.

* Corresponding author. Mailing address: Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Av. Moraes Rêgo s/n, Cidade Universitária, CEP 50670-420, Recife-PE, Brazil. Phone: 55-81-33012500. Fax: 55-81-4532449. E-mail: yara{at}cpqam.fiocruz.br.

Clinical and Diagnostic Laboratory Immunology, September 2001, p. 1024-1027, Vol. 8, No. 5
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.5.1024-1027.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.