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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 847-849, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.847-849.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Production of Bartonella Genus-Specific Monoclonal Antibodies

Zhongxing Liang, Bernard La Scola, Hubert Lepidi, and Didier Raoult*

Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Mediterranée, 13385 Marseille Cédex, France

Received 26 October 2000/Returned for modification 22 January 2001/Accepted 3 April 2001

Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 different Bartonella species were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted with Chlamydia psittaci and Chlamydia trachomatis at low titers. The identification of a common Bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it in Escherichia coli. Moreover, these MAbs allow all Bartonella species to be identified to the genus level.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 Blvd. Jean Moulin, 13385 Marseille Cédex 5, France. Phone: (33) 4 91 32 43 75. Fax: (33) 4 91 83 03 90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 847-849, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.847-849.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.