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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 837-840, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.837-840.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cytopathic Changes in Rat Microglial Cells Induced by Pathogenic Acanthamoeba culbertsoni: Morphology and Cytokine Release

Ho-Joon Shin,1,* Myung-Soo Cho,1 Suk-Yul Jung,1 Hyung-Il Kim,1 Sun Park,1 Jang-Hoon Seo,2 Jung-Chil Yoo,3 and Kyung-Il Im4

Department of Microbiology, Ajou University School of Medicine, Suwon 442-749,1 Department of Clinical Pathology, College of Shinheung, Uejongbu 480-701,2 Department of Biology, College of Natural Science, Kyung-Hee University, Seoul 130-701,3 and Department of Parasitology, Yonsei University, College of Medicine, Seoul 121-752,4 Korea

Received 22 December 2000/Returned for modification 13 April 2001/Accepted 2 May 2001

To determine whether pathogenic Acanthamoeba culbertsoni trophozoites and lysate can induce cytopathic changes in primary-culture microglial cells, morphological changes were observed by transmission electron microscopy (TEM). In addition, the secretion of two kinds of cytokines, tumor necrosis factor alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ), from microglial cells was observed. Trophozoites of pathogenic A. culbertsoni made contact with microglial cells and produced digipodia. TEM revealed that microglial cells cocultured with amoebic trophozoites underwent a necrotic process, accompanied by lysis of the cell membrane. TEM of microglial cells cocultured with amoebic lysate showed that the membranes of the small cytoplasmic vacuoles as well as the cell membrane were lysed. The amounts of TNF-alpha secreted from microglial cells cocultured with A. culbertsoni trophozoites or lysate increased at 6 h of incubation. The amounts of IL-1beta secreted from microglial cells cocultured with A. culbertsoni trophozoites at 6 h of incubation was similar to those secreted from the control group, but the amounts decreased during cultivation with A. culbertsoni lysate. These results suggest that pathogenic A. culbertsoni induces the cytopathic effects in primary-culture rat microglial cells, with the effects characterized by necrosis of microglial cells and changes in levels of secretion of TNF-alpha and IL-1beta from microglial cells.

* Corresponding author. Mailing address: Department of Microbiology, Ajou University School of Medicine, Suwon 442-749, Korea. Phone: (82) 31-219-5076. Fax: (82) 32-219-5079. E-mail: hjshin{at}madang.ajou.ac.kr.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 837-840, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.837-840.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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