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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 828-831, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.828-831.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Real-Time PCR as a New Tool for Quantifying Leishmania infantum in Liver in Infected Mice

Stéphane Bretagne,1,* Rémy Durand,2 Martine Olivi,3 Jean-François Garin,4 Annie Sulahian,4 Danièle Rivollet,1 Michel Vidaud,3 and Michèle Deniau1

Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor-APHP and Université Paris XII, Créteil,1 and Laboratoire de Parasitologie-Mycologie, Hôpital Bichat-APHP,2 Laboratoire de Génétique Moléculaire, Faculté de Pharmacie,3 and Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis-APHP and Université Paris VI,4 Paris, France.

Received 20 November 2000/Returned for modification 9 January 2001/Accepted 19 March 2001

The parasitic loads of mouse livers experimentally infected with Leishmania infantum were determined using a double real-time quantitative PCR test targeted to the parasite DNA polymerase gene and to the mouse brain-derived neutrophic factor gene. The Leishmania DNA copy number was normalized to the number of mouse gene copies in order to quantify the former independently of liver weight. The correlation coefficient with the microtitration method was 0.66. This PCR assay can be considered for experimental pharmaceutical studies.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, 51 avenue du Général DeLattre de Tassigny, 94010, Créteil, Cedex, France. Phone: 33 1 49 81 28 90. Fax: 33 1 49 81 36 01. E-mail: bretagne{at}univ-paris12.fr.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 828-831, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.828-831.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.