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Clinical and Diagnostic Laboratory Immunology, March 2001, p. 429-431, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.429-431.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multicenter Evaluation of a Rapid and Convenient Method for Determination of Cytomegalovirus Immunoglobulin G Avidity

Monique Baccard-Longere,1,* Francois Freymuth,2 Denis Cointe,3 Jean Marie Seigneurin,1 and Liliane Grangeot-Keros3

Laboratoire de Virologie, Centre Hospitalier Universitaire de Grenoble, Grenoble,1 Laboratoire de Virologie, Centre Hospitalier Universitaire de Caen, Caen,2 and Laboratoire de Virologie et d'Immunologie, Hopital Antoine-Beclere, Clamart,3 France

Received 14 August 2000/Returned for modification 17 November 2000/Accepted 2 January 2001

An easy, rapid, and reproducible test to distinguish residual cytomegalovirus (CMV) immunoglobulin M (IgM) antibodies from antibodies produced in primary infection could be useful, especially for pregnant women. The CMV avidity of IgG antibodies with the VIDAS automated enzyme-linked fluorescent assay and 6 M urea was evaluated in a multicenter study to differentiate between primary CMV infections and past infections or reactivations. A total of 416 serum specimens were tested: 159 specimens were from follow-up of primary infections, and 257 were from past infections. All of the specimens from primary infections collected within 4 months (17 weeks) after the onset of the infection had an avidity index lower than 0.8. An avidity index higher than 0.8 excludes a recent primary infection of less than 4 months. However, an avidity index higher than 0.8 cannot confirm all past infections, since 48 specimens (18%) from past infections had an avidity index lower than 0.8 (between 0.5 and 0.8). The exclusion capacity could be improved (96.9%) by using a cutoff of 0.7, but this index would decrease the specificity of the technique, since the avidity index was found to be between 0.7 and 0.8 in two patients with recent primary infection. All specimens from primary infections obtained more than 4 months after the onset of infection had an avidity index more than 0.2. In this study, an avidity index less than 0.2 confirms the presence of a recent primary infection of less than 4 months. The VIDAS CMV IgG avidity test is a rapid, reproducible test with very good performance.

* Corresponding author. Mailing address: Laboratoire de Virologie, Centre Hospitalier Universitaire de Grenoble, BP 217, 38043 Grenoble, France. Phone: (33) 4 76 76 56 04. Fax: (33) 4 76 76 52 28. E-mail: MBaccard{at}chu-grenoble.fr.

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 429-431, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.429-431.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.