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Clinical and Diagnostic Laboratory Immunology, March 2001, p. 415-423, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.415-423.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Enzyme Immunoassay Detection of Antigen-Specific Immunoglobulin G Antibodies in Longitudinal Serum Samples from Patients with Cryptosporidiosis

Jeffrey W. Priest,1,* Anna Li,2 Mohamad Khan,2 Michael J. Arrowood,1 Patrick J. Lammie,1 Corinne S. Ong,2 Jacquelin M. Roberts,1 and Judith Isaac-Renton2

Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724,1 and Department of Pathology and Laboratory Medicine, University of British Columbia and British Columbia Centre for Disease Control Laboratory Services, Vancouver, British Columbia V5Z 4R4, Canada2

Received 3 October 2000/Returned for modification 11 December 2000/Accepted 9 January 2001

Cryptosporidium parvum is a protozoan parasite that causes diarrheal illness in a wide range of mammalian hosts, including humans. Characteristic serum immunoglobulin G (IgG) antibody responses to antigens in the 27- and 17-kDa size ranges have been shown to develop after infection, and several enzyme-linked immunosorbent assay (ELISA) and Western blot assay formats have been used to measure these IgG levels in human serum. Using a collection of serial samples from laboratory-confirmed cryptosporidiosis patients, we compared the results obtained by using two new ELISAs with those obtained with two different Western blot assays. When assayed with the large-format Western blot, 97% of the 67 patients had a demonstrable antibody response on at least one occasion. The Cp23 ELISA correctly identified 93% of the samples that had a 27-kDa response by Western blot and 100% of the negative samples. The Triton antigen ELISA detected 77% of the samples that had a 17-kDa response by Western blot and 88% of the negative samples. The sensitivity of the Triton antigen assay was higher for samples collected between 16 and 92 days after the onset of symptoms (96%). The minigel-format Western blot did not compare favorably with the large-format blot for the detection of antibodies to the 27-kDa antigen (71% sensitivity). A half-life of about 12 weeks was estimated for antibodies to both the 27- and 17-kDa antigens. We believe the Cp23 and Triton antigen ELISAs will be useful in epidemiologic studies of the prevalence of Cryptosporidium infection in the population.

* Corresponding author. Mailing address: Division of Parasitic Diseases, Centers for Disease Control and Prevention, Mail Stop F-13, Building 23, Room 1025, 4770 Buford Highway N.E., Atlanta, GA 30341-3724. Phone: (770) 488-4587. Fax: (770) 488-4108. E-mail: jpriest{at}cdc.gov.

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 415-423, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.415-423.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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