CVI
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Inokuma, H.
Right arrow Articles by Brouqui, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Inokuma, H.
Right arrow Articles by Brouqui, P.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 241-244, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.241-244.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analysis of the 16S rRNA Gene Sequence of Anaplasma centrale and Its Phylogenetic Relatedness to Other Ehrlichiae

Hisashi Inokuma,1,2 Yutaka Terada,3 Tugihiko Kamio,3 Didier Raoult,2 and Philippe Brouqui2,*

Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515,1 and National Institute of Animal Health, Tsukuba 305-0856,3 Japan, and Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille, France2

Received 31 August 2000/Returned for modification 19 October 2000/Accepted 20 November 2000

The nucleotide sequence of the Anaplasma centrale 16S rRNA gene was determined and compared with the sequences of ehrlichial bacteria. The sequence of A. centrale was closely related to Anaplasma marginale by both level-of-similarity (98.08% identical) and distance analysis. A species-specific PCR was developed based upon the alignment data. The PCR can detect A. centrale DNA extracted from 10 infected bovine red blood cells in a reaction mixture. A. centrale DNA was amplified in the reaction, but not other related ehrlichial species.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 bd. Jean Moulin, 13385 Marseille Cédex 5, France. Phone: (33)-4-91-32-43-75. Fax: (33)-4-91-83-03-90. E-mail: Philippe.Brouqui{at}medecine.univ-mrs.fr

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 241-244, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.241-244.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.