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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 199-202, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.199-202.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Diagnosis of Granulocytic Ehrlichiosis in Humans by Immunofluorescence Assay

P. Brouqui,1,* E. Salvo,1 J. S. Dumler,2 and D. Raoult1

Unité des rickettsies, WHO Collaborative Center for Rickettsial Reference and Research, CNRS UPRES A 6020, Faculté de Médecine, 13385 Marseille Cedex 5 France, 1 and Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland2

Received 19 July 2000/Returned for modification 16 August 2000/Accepted 10 October 2000

Serodiagnostic tests are widely available for tick-borne diseases. We evaluated a cell-free antigen of the human granulocytic ehrlichiosis agent. Immunofluorescence assay (IFA) with this antigen is as efficient as with the MRL kit and allows a one-step IFA with other cell-free antigens that is useful when testing sera from patients bitten by ticks.

* Corresponding author. Mailing address: Unité des rickettsies, WHO Collaborative Center for Rickettsial Reference and Research, CNRS UPRES A 6020, Faculté de Médecine, 27 bd Jean Moulin, 13385 Marseille Cedex 5, France, Phone: 33 4 91 32 43 75. Fax: 33 4 91 83 03 90. E-mail: Philippe.Brouqui{at}medecine.univ-mrs.fr.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 199-202, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.199-202.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.