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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 170-173, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.170-173.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Specific Serological Assays for Diagnosing Human Herpesvirus 6 Infection after Liver Transplantation

Tetsushi Yoshikawa,1,* Jodi B. Black,2 Masaru Ihira,1 Kyoko Suzuki,1 Sadao Suga,1 Keiji Iida,3 Yumiko Saito,3 Katsuhiro Asonuma,4 Koichi Tanaka,4 and Yoshizo Asano1

Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi,1 Special Reference Laboratories Inc., Tokyo,3 and Department of Transplantation and Immunology, Kyoto University School of Medicine, Kyoto,4 Japan, and National Cancer Institute, National Institutes of Health, Bethesda, Maryland2

Received 3 April 2000/Returned for modification 18 August 2000/Accepted 18 September 2000

Cross-reactivity between human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) antibodies and the reliability of specific serological assays were analyzed for 12 patients with concurrent HHV-6 and HHV-7 antibody responses after transplantation with a liver from a living relative by using an immunofluorescence assay (IFA). A neutralizing antibody titer assay (NT) and an immunoblot assay (IB) designed to detect immunoglobulin M (IgM) antibody to the HHV-6 immunodominant 101-kDa protein were compared in the diagnosis of an active HHV-6 infection. A total of 9 of 12 patients demonstrated concurrent HHV-6 and HHV-7 antibody responses, including increased IgG titers and/or the presence of IgM by IFA, and were thus analyzed for cross-reactive antibody to heterologous virus. The average percentages of residual antibody to HHV-6 and HHV-7 after absorption with HHV-6 antigen were 32.6% (range, 6 to 50%) and 55.6% (range, 35 to 100%), respectively. All 12 patients were subsequently analyzed for HHV-6 antibody by using IB and NT. IB detected IgM antibody to the 101-kDa protein in 75% (9 of 12) of the recipients. A significant rise in the NT antibody titer was detected in the same nine samples. However, HHV-6 DNA was detected by PCR in only five of nine plasma samples collected from recipients with a specific serologic response against HHV-6.

* Corresponding author. Mailing address: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 4668550, Japan. Phone: 8 1 -52-7442451. Fax: 8 1 -52-7442452. E-mail: tetsushi{at}med.nagoya-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 170-173, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.170-173.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.