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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 166-169, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.166-169.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis

Henk L. Smits,1,* C. K. Eapen,2 Sheela Sugathan,2 Mariamma Kuriakose,2 M. Hussein Gasem,3 Claude Yersin,4 David Sasaki,5 Bambang Pujianto,3 Marc Vestering,1 Theresia H. Abdoel,1 and George C. Gussenhoven1

Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands1; Malankara Orthodox Syrian Church Medical Mission Hospital, Kolenchery, India2; Department of Medicine, Dr. Kariadi Hospital/Diponegoro University, Semarang, Indonesia3; Department of Medicine, Victoria Hospital, The Seychelles4; and Epidemiology Branch, Department of Health, Honolulu, Hawaii5

Received 12 June 2000/Returned for modification 20 September 2000/Accepted 19 October 2000

An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.

* Corresponding author. Mailing address: Department of Biomedical Research, Royal Tropical Institute, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. Phone: 31 20 5665470. Fax: 31 20 6971841. E-mail: H.Smits{at}kit.nl.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 166-169, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.166-169.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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