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Clinical and Diagnostic Laboratory Immunology, September 2000, p. 850-852, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of a Capture Screening Enzyme-Linked Immunosorbent Assay for Combined Determination of Immunoglobulin M and G Antibodies Produced during Dengue Infection

Sai Kit Lam,1,* Cheng Lan Ew,1 Jody L. Mitchell,2 Andrea J. Cuzzubbo,2 and Peter L. Devine2

WHO Collaborating Centre for Arbovirus Reference and Research (Dengue and Dengue Haemorrhagic Fever), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia,1 and PanBio Pty Ltd, Windsor 4030, Brisbane, Queensland, Australia2

Received 14 March 2000/Returned for modification 8 June 2000/Accepted 12 July 2000

A commercially available enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Screening ELISA) that utilized both immunoglobulin M (IgM) and IgG capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%.

* Corresponding author. Mailing address: WHO Collaborating Centre for Arbovirus Reference and Research (Dengue and Dengue Haemorrhagic Fever), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Phone: 60 3-7502324. Fax: 60 3-7582801. E-mail: lamsk{at}medicine.med.um.edu.my.

Clinical and Diagnostic Laboratory Immunology, September 2000, p. 850-852, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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Copyright © 2000 by the American Society for Microbiology. All rights reserved.