CVI
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nawa, M.
Right arrow Articles by Kurane, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nawa, M.
Right arrow Articles by Kurane, I.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, September 2000, p. 774-777, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Serotype-Cross-Reactive Immunoglobulin M Responses in Dengue Virus Infections Determined by Enzyme-Linked Immunosorbent Assay

Masaru Nawa,1,* Ken-Ichiro Yamada,2 Tomohiko Takasaki,2 Toshitaka Akatsuka,1 and Ichiro Kurane2

Department of Microbiology, Saitama Medical School, Saitama 350-0495,1 and Department of Virology 1, National Institute of Infectious Diseases, Shinjyuku, Tokyo 162-8640,2 Japan

Received 1 February 2000/Returned for modification 4 April 2000/Accepted 31 May 2000

We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.

* Corresponding author. Mailing address: Department of Microbiology, Saitama Medical School, 38 Moroyama, Saitama 350-0495, Japan. Phone: 81 492 76 1166. Fax: 81 492 95 9107. E-mail: mnawa{at}saitama-med.ac.jp.

Clinical and Diagnostic Laboratory Immunology, September 2000, p. 774-777, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 2000 by the American Society for Microbiology. All rights reserved.