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Clinical and Diagnostic Laboratory Immunology, July 2000, p. 574-577, Vol. 7, No. 4
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antibody to Heat Shock Protein Can Be Used for Early Serological Monitoring of Helicobacter pylori Eradication Treatment

Naoko Yunoki,1 Kenji Yokota,2 Motowo Mizuno,1 Yoshiro Kawahara,1 Masayasu Adachi,1 Hiroyuki Okada,1 Shyunji Hayashi,3 Yoshikazu Hirai,3 Keiji Oguma,2,* and Takao Tsuji1

First Department of Internal Medicine1 and Department of Bacteriology,2 Okayama University Medical School, 2-5-1 Shikata-cho Okayama 700-8558, and Department of Microbiology, Jichi Medical School, 3311-01 Yakushiji Minami-kouch Tochigi 329-0431,3 Japan

Received 5 November 1999/Returned for modification 8 February 2000/Accepted 7 April 2000

Infection with Helicobacter pylori induces humoral immune responses against various antigens of the bacterium. Heat shock proteins (hsps) are immunodominant antigens in various diseases including H. pylori infection. In the present study, we measured the anti-hsp antibody titers in 42 patients with H. pylori-infected peptic ulcers during a bacterial eradication study. The patients were treated with a proton pump inhibitor and antimicrobial agents to eradicate the organism. Their sera were obtained at pretreatment and at 1 month and 6 months after the eradication therapy. The titers of immunoglobulin G antibodies to the H. pylori hsp, whole-cell lysate, and urease (30-kDa subunit) antigens in serum were measured by a capture enzyme-linked immunosorbent assay. The levels of H. pylori hsp60 antibodies in sera collected 1 month after treatment had declined significantly, even when changes in the titers of antibodies to whole-cell and urease antigens were not apparent. These results suggest that measurement of antibodies to H. pylori hsp60 in serum is useful for the early monitoring of the effectiveness of eradication therapy.

* Corresponding author. Mailing address: Department of Bacteriology, Okayama University, School of Medicine, 2-5-1 Shikata, Okayama 700-8558, Japan. Phone: 81-86-235-7157. Fax: 81-86-235-7162. E-mail: kuma{at}med.okayama-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, July 2000, p. 574-577, Vol. 7, No. 4
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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