Previous Article | Next Article ![]()
Clinical and Diagnostic Laboratory Immunology, July 2000, p. 549-552, Vol. 7, No. 4
Departments of Medical
Microbiology1 and Image
Diagnosis,3 Clinic Hospital of Valencia,
University of Valencia, and Department of Statistics and
Operation Research, University of Valencia,2
Valencia, Spain
Received 5 January 2000/Returned for modification 17 February
2000/Accepted 23 March 2000
A discriminant technique was applied to the different serological
patterns obtained by enzyme-linked immunoelectrotransfer blotting
(EITB) and by conventional immunological tests, in order to
differentiate the residual antibody patterns present in healed hydatidosis from the ones present in patients with active hydatidosis. For this purpose, specific antibodies against Echinococcus
granulosus were detected by indirect hemagglutination,
agglutination of latex particles, basophil degranulation, and EITB for
23 patients with active hydatidosis and 45 patients with surgically
cured hydatidosis. Discriminant analysis of the different serological
patterns obtained by EITB and conventional serology correctly
classified 92.54% of patients (93.3% if the patients are
differentiated according to the time elapsed since surgery). This
method detected the presence of active hydatidosis in 95.6% of
patients for whom abdominal ultrasonography had confirmed the presence
of active hydatid cysts. The global specificity was 88.9%. The
specificity was 97.1% for patients who had been operated on 3 years
ago or more and 63.6% for patients with less time since surgery.
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Immunological Diagnosis of Human Hydatid Cyst Relapse:
Utility of the Enzyme-Linked Immunoelectrotransfer Blot and
Discriminant Analysis

and
*
Corresponding author. Present address: Departamento de
Microbiología Médica de la Fundación Jiménez
Díaz, Avenida Reyes Católicos 2, 28040 Madrid, Spain.
Phone: 34 91 550 49 00. Fax: 34 91 549 47 64. E-mail:
igadea{at}fjd.es.
Present address: Departamento de Radiodiagnóstico, Hospital
Luis Alcañiz, Játiva (Valencia), Spain.
| Antimicrob. Agents Chemother. | Clin. Microbiol. Rev. | Infect. Immun. |
|---|---|---|
| J. Clin. Microbiol. | J. Virol. | ALL ASM JOURNALS |