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Clinical and Diagnostic Laboratory Immunology, May 2000, p. 507-509, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Clonal Analysis of the Human B-Cell Repertoire Using a Heteromobility Assay

Deepanker Tewari*

Aaron Diamond AIDS Center, New York, New York 10016

Received 20 September 1999/Returned for modification 24 November 1999/Accepted 27 January 2000

A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.

* Present address: Microbiology Department, Bureau of Animal Health/Diag Services, Harrisburg, PA 17110. Phone: (717) 787-8808. Fax: (717) 772-3895. E-mail: dtewari{at}state.pa.us.

Clinical and Diagnostic Laboratory Immunology, May 2000, p. 507-509, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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Copyright © 2000 by the American Society for Microbiology. All rights reserved.