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Clinical and Diagnostic Laboratory Immunology, May 2000, p. 451-456, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of 12 Commercial Tests for Detection of Epstein-Barr Virus-Specific and Heterophile Antibodies

Anne-Lise Bruu,1,* Reidar Hjetland,2 Ellen Holter,3 Liisa Mortensen,4 Olav Natås,5 Wenche Petterson,6 Anne Grete Skar,7 Tone Skarpaas,8 Trygve Tjade,9 and Birgitta Åsjø10

Department of Virology, National Institute of Public Health, Oslo,1 Department of Microbiology, Sogn and Fjordane Central Hospital, Førde,2 Institute of Microbiology, The National Hospital, Oslo,3 Department of Microbiology, Nordland Central Hospital, Bodø,4 Department of Microbiology, Rogaland Central Hospital, Stavanger,5 Laboratory of Microbiology, Vestfold Central Hospital, Tønsberg,6 Department of Microbiology, Ullevål University Hospital, Oslo,7 Department of Microbiology, Vest-Agder Central Hospital, Kristiansand,8 Department of Microbiology, Akershus Central Hospital, Nordbyhagen,9 and Department of Microbiology and Immunology, Haukeland University Hospital, Bergen,10 Norway

Received 25 October 1999/Returned for modification 5 January 2000/Accepted 25 February 2000

Ten microbiological departments in Norway have participated in a multicenter evaluation of the following commercial tests for detection of Epstein-Barr virus (EBV)-specific and heterophile antibodies: CAPTIA Select viral capsid antigen (VCA)-M/G/EBNA (Centocor Inc.), Enzygnost anti-EBV/immunoglobulin M (IgM) and IgG (Dade Behring), Vironostika EBV VCA IgM/IgG/EBNA enzyme-linked immunosorbent assay (ELISA) (Organon Teknika), SEROFLUOR immunofluorescence assay and EBV Combi-Test (Institute Virion Ltd.), anti-EBV recombinant IgM- and IgG-early antigen/EBNA IgG ELISA (Biotest Diagnostics), EBV IgM/IgG/EBNA ELISA (Gull Laboratories), Paul-Bunnell-Davidsohn test (Sanofi Diagnostics Pasteur), Monosticon Dri-Dot (Organon Teknika), Avitex-IM (Omega Diagnostics Ltd.), Alexon Serascan infectious mononucleosis test (Alexon Biomedical Inc.), Clearview IM (Unipath Ltd.), and Cards±OS Mono (Pacific Biotech, Inc.). The test panel included sera from patients with primary EBV infection, immunocompromised patients with recent cytomegalovirus infection, healthy persons (blood donors), and EBV-seronegative persons. Among the tests for EBV-specific antibodies the sensitivity was good, with only small differences between the different assays. However, there was a greater variation in specificity, which varied between 100% (Enzygnost) and 86% (Biotest). Tests for detection of heterophile antibodies based on purified or selected antigen (Avitex, Alexon, Clearview IM, and Cards±OS Mono) were more sensitive than the Paul-Bunnell-Davidsohn and Monosticon tests.

* Corresponding author. Mailing address: Department of Virology, National Institute of Public Health, Postbox 4404 Torshov, 0403 Oslo, Norway. Phone: 47 22 04 23 12. Fax: 47 22 04 24 47. E-mail: anne-lise.bruu{at}folkehelsa.no.

Clinical and Diagnostic Laboratory Immunology, May 2000, p. 451-456, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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