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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 312-313, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Clinical Application of a Dot Blot Test for Diagnosis of Enteric Fever Due to Salmonella enterica Serovar Typhi in Patients with Typhoid Fever from Colombia and Peru

Nora Cardona-Castro,1,* Eduardo Gotuzzo,2 Monica Rodriguez,1 and Humberto Guerra2

Instituto Columbiano de Medicina Tropical, AA 52162 Medellín, Colombia,1 and Hospital Cayetano Heredia---Instituto de Medicina Tropical Alexander von Humbolt, Lima 100, Perú2

Received 27 September 1999/Returned for modification 5 November 1999/Accepted 21 December 1999

Clinical application of a dot blot test to detect immunoglobulin G (IgG) (88% sensitivity and specificity) and IgM (12.1% sensitivity and 97% specificity) against flagellar antigen from Salmonella enterica serovar Typhi was performed in Peruvian and Colombian patients with typhoid fever. This test can be used as a good predictor of serovar Typhi infection in regions lacking laboratory facilities and in field studies.

* Corresponding author. Mailing address: Instituto Colombiano de Medicina Tropical, Cra 51a No. 62-42, AA 52162 Medellín, Columbia. Phone: (574) 211-7788. Fax: (574) 571-6960. E-mail: icmt{at}epm.net.co.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 312-313, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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