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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 125-128, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of Positive Selectable Markers for the Fungal Pathogen Cryptococcus neoformans

Jun Hua, Justin D. Meyer, and Jennifer K. Lodge*

Edward A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, Missouri 63104

Received 30 August 1999/Returned for modification 14 October 1999/Accepted 27 October 1999

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningitis in ~10% of patients with AIDS. New selectable markers which confer resistance to G418 or phleomycin when transformed into C. neoformans were made. A hygromycin-selectable marker was modified to allow selection with a single copy of the marker.


* Corresponding author. Mailing address: Edward A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, 1402 S. Grand Blvd., St. Louis, MO 63104. Phone: (314) 577-8143. Fax: (314) 577-8156. E-mail: lodgejk{at}slu.edu.


Clinical and Diagnostic Laboratory Immunology, January 2000, p. 125-128, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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