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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 125-128, Vol. 7, No. 1
Edward A. Doisy Department of Biochemistry
and Molecular Biology, St. Louis University School of Medicine, St.
Louis, Missouri 63104
Received 30 August 1999/Returned for modification 14 October
1999/Accepted 27 October 1999
Cryptococcus neoformans is an opportunistic fungal
pathogen that causes meningitis in ~10% of patients with AIDS. New
selectable markers which confer resistance to G418 or phleomycin when
transformed into C. neoformans were made. A
hygromycin-selectable marker was modified to allow selection with a
single copy of the marker.
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Development of Positive Selectable Markers for the
Fungal Pathogen Cryptococcus neoformans
*
Corresponding author. Mailing address: Edward A. Doisy
Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, 1402 S. Grand Blvd., St. Louis, MO 63104. Phone:
(314) 577-8143. Fax: (314) 577-8156. E-mail:
lodgejk{at}slu.edu.
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