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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 1-5, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sonicated Diagnostic Immunoblot for Bartonellosis

Vania Mallqui,1 Emily C. Speelmon,1,2 Manuela Verástegui,1,3 Ciro Maguiña-Vargas,4 Paula Pinell-Salles,2 Rosa Lavarello,2 Jose Delgado,2 Margaret Kosek,5 Sofia Romero,6 Yanina Arana,1 and Robert H. Gilman1,2,7,*

Departamento de Patología,1 Departamento de Microbiología,3 and Instituto de Medicina Tropical Alexander VonHumboldt,4 Universidad Peruana Cayetano Heredia, Asociación Benéfica PRISMA,2 and United States Naval Medical Research Institute Detachment (NAMRID),6 Lima Peru; Department of Internal Medicine, Harbor-UCLA Medical Center, Torrance, California5; and Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland7

Received 26 May 1999/Returned for modification 25 June 1999/Accepted 14 September 1999

Two simple Bartonella bacilliformis immunoblot preparation methods were developed. Antigen was prepared by two different methods: sonication of whole organisms or glycine extraction. Both methods were then tested for sensitivity and specificity. Well-defined control sera were utilized in the development of these diagnostic immunoblots, and possible cross-reactions were thoroughly examined. Sera investigated for cross-reaction with these diagnostic antigens were drawn from patients with brucellosis, chlamydiosis, Q fever, and cat scratch disease, all of whom were from regions where bartonellosis is not endemic. While both immunoblots yielded reasonable sensitivity and high specificity, we recommend the use of the sonicated immunoblot, which has a higher sensitivity when used to detect acute disease and produces fewer cross-reactions. The sonicated immunoblot reported here is 94% sensitive to chronic bartonellosis and 70% sensitive to acute bartonellosis. In a healthy group, it is 100% specific. This immunoblot preparation requires a simple sonication protocol for the harvesting of B. bacilliformis antigens and is well suited for use in regions of endemicity.

* Corresponding author. Mailing address: Department of International Health, Johns Hopkins University School of Hygiene and Public Health, 615 N. Wolfe St., Room 3501/3503, Baltimore, MD 21205. Phone: (410) 614-3959. Fax: (410) 614-6060. E-mail: rgilman{at}jhpsh.edu.

Clinical and Diagnostic Laboratory Immunology, January 2000, p. 1-5, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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