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Clinical and Diagnostic Laboratory Immunology, September 1999, p. 729-733, Vol. 6, No. 5
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cell Culture of Sporadic Hepatitis E Virus in China

Rutong Huang,1,* Derong Li,1 Shaojing Wei,2 Qinghong Li,1 Xitong Yuan,1 Liqing Geng,1 Xiaoyu Li,1 and Minxia Liu1

Beijing Institute of Microbiology and Epidemiology, Beijing 100850,1 and Institute of Infections Diseases, Guangzhou Municipal Infectious Diseases Hospital, Guangzhou 510060,2 China

Received 18 November 1998/Returned for modification 28 January 1999/Accepted 25 May 1999

The isolation and identification of the 87A strain of epidemic hepatitis E virus (HEV) by means of cell culturing have been described previously. This paper reports the successful isolation of a sporadic HEV strain (G93-2) in human lung carcinoma cell (A549) cultures. The etiology, molecular and biological properties, and serological relationship of this new strain to other, epidemic HEV strains are described. The propagation of both sporadic and epidemic HEV strains in a cell culture system will facilitate vaccine research.

* Corresponding author. Mailing address: Beijing Institute of Microbiology and Epidemiology, Beijing 100850, China. Phone: (8610)66931535. Fax: (8610)68213044. E-mail: Luqj{at}nic.bmi.ac.cn.

Clinical and Diagnostic Laboratory Immunology, September 1999, p. 729-733, Vol. 6, No. 5
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 1999 by the American Society for Microbiology. All rights reserved.