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Clinical and Diagnostic Laboratory Immunology, July 1999, p. 499-503, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cell Surface Display of Human Immunodeficiency Virus Type 1 gp120 on Escherichia coli by Using Ice Nucleation Protein

Young-Don Kwak, Seung-Ku Yoo,* and Eui-Joong Kim

Department of Biotechnology, College of Engineering and Bioproduct Research Center, Yonsei University, 134 Shinchon-Dong, Sudaemun-Ku, Seoul 120-749, Korea

Received 21 September 1998/Returned for modification 6 February 1999/Accepted 8 April 1999

A new system designed for cell surface display of recombinant proteins on Escherichia coli has been evaluated for expression of eukaryotic viral proteins. Human immunodeficiency virus type 1 (HIV-1) gp120 was fused to the C terminus of ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae. Western blotting, immunofluorescence microscopy, fluorescence-activated cell-sorting analysis, whole-cell enzyme-linked immunosorbent assay, and ice nucleation activity assay confirmed the successful expression of HIV-1 gp120 on the surface of Escherichia coli. This study shows that the INP system can be used for the expression of eukaryotic viral proteins. There is also a possibility that the INP system can be used as an AIDS diagnostic system, an oral vaccine delivery system, and an expression system for various heterologous higher-molecular-weight proteins.

* Corresponding author. Mailing address: Department of Biotechnology, College of Engineering and Bioproduct Research Center, Yonsei University, 134 Shinchon-Dong, Sudaemun-Ku, Seoul, 120-749, Korea. Phone: 82-2-361-2887. Fax: 82-2-362-7265. E-mail: skyoo{at}bubble.yonsei.ac.kr.

Clinical and Diagnostic Laboratory Immunology, July 1999, p. 499-503, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.