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Clinical and Diagnostic Laboratory Immunology, May 1999, p. 434-436, Vol. 6, No. 3
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Estimation of Growth Rates of Escherichia coli BJ4 in Streptomycin-Treated and Previously Germfree Mice by In Situ rRNA Hybridization

Camilla U. Rang,1 Tine Rask Licht,2,3 Tore Midtvedt,4 Patricia L. Conway,5 Lin Chao,6 Karen A. Krogfelt,3 Paul S. Cohen,7 and Søren Molin2,*

Department for General and Marine Microbiology, Göteborg University, Göteborg,1 and Department for Medical Microbial Ecology, Karolinska Institute, Stockholm,4 Sweden; Department for Microbiology, The Technical University of Denmark, Lyngby,2 and Department of Gastrointestinal Infections, Statens Seruminstitut, Copenhagen,3 Denmark; School of Microbiology and Immunology, University of New South Wales, Kensington, Australia5; Department of Zoology, University of Maryland, College Park, Maryland6; and Department of Biochemistry, Microbiology, and Molecular Genetics, University of Rhode Island, Kingston, Rhode Island7

Received 10 August 1998/Returned for modification 19 January 1999/Accepted 24 February 1999

The growth physiology of Escherichia coli during colonization of the intestinal tract was studied with four animal models: the streptomycin-treated mouse carrying a reduced microflora, the monoassociated mouse with no other microflora than the introduced strain, the conventionalized streptomycin-treated mouse, and the conventionalized monoassociated mouse harboring a full microflora. A 23S rRNA fluorescent oligonucleotide probe was used for hybridization to whole E. coli cells fixed directly after being taken from the animals, and the respective growth rates of E. coli BJ4 in the four animal models were estimated by correlating the cellular concentrations of ribosomes with the growth rate of the strain. The growth rates thus estimated from the ribosomal content of E. coli BJ4 in vivo did not differ in the streptomycin-treated and the monoassociated mice. After conventionalization there was a slight decrease of the bacterial growth rates in both animal models.

* Corresponding author. Mailing address: Department of Microbiology, The Technical University of Denmark, Building 301, 2800 Lyngby, Denmark. Phone: 45 45 25 25 13. Fax: 45 45 93 28 09. E-mail: sm{at}im.dtu.dk.

Clinical and Diagnostic Laboratory Immunology, May 1999, p. 434-436, Vol. 6, No. 3
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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