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Clinical and Diagnostic Laboratory Immunology, May 1999, p. 429-433, Vol. 6, No. 3
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Production, Characterization, and Epitope Mapping of a Monoclonal Antibody against Aspartic Proteinase of Candida albicans

Byoung-Kuk Na,1 Gyung-Tae Chung,2 and Chul-Yong Song1,*

Department of Biology and Institute of Basic Science, College of Natural Science, Chung-Ang University, Seoul 156-756,1 and Department of Bacterial Disease, National Institute of Health, Seoul 122-701,2 Korea

Received 21 July 1998/Returned for modification 15 October 1998/Accepted 12 January 1999

A monoclonal antibody (MAb; MAb CAP1) that was reactive with extracellular aspartic proteinase of Candida albicans (CAP) was produced. The MAb showed strong sensitivity and reactivity to CAP but not to the aspartic proteinases of Candida parapsilosis, Candida tropicalis, and Aspergillus fumigatus or to human cathepsin D or porcine pepsin. The epitope of the CAP recognized by the MAb was the proteinaseous part of CAP and the putative epitope of the MAb was located in the Asp77 to Gly103 sequence. This antibody could be useful for the characterization of CAP and would be a valuable probe for the detection of CAP antigen in the sera of patients with invasive candidiasis.

* Corresponding author. Mailing address: Department of Biology, College of Natural Science, Chung-Ang University, 221 Heuksukdong, Dongjakgu, Seoul 156-756, Korea. Phone: 82-2-820-5208. Fax: 82-2-816-6710. E-mail: cysong{at}cau.ac.kr.

Clinical and Diagnostic Laboratory Immunology, May 1999, p. 429-433, Vol. 6, No. 3
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.