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Clinical and Diagnostic Laboratory Immunology, January 1999, p. 130-132, Vol. 6, No. 1
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assaydagger

S. L. SchoenthalerDagger and S. Kapil*

Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606

Received 3 June 1998/Returned for modification 17 August 1998/Accepted 30 September 1998

We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 104 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.

* Corresponding author. Mailing address: Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4457. Fax: (785) 532-4039. E-mail: kapil{at}vet.ksu.edu.

dagger Contribution 98-458-J from the Kansas Agricultural Experiment Section.

Dagger Present address: University of Kansas Medical Center, Kansas City, KS 66103.

Clinical and Diagnostic Laboratory Immunology, January 1999, p. 130-132, Vol. 6, No. 1
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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