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Clinical and Diagnostic Laboratory Immunology, November 1998, p. 745-748, Vol. 5, No. 6
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Rapid Flow Cytometric Method to Explore Cellular Immunity against Toxoplasma gondii in Humans

Sandrine Kahi,1,2 Grégoire J. N. Cozon,1,3,* Timothy Greenland,4 Martine Wallon,1,2 Françoise Gay-Andrieu,1,2 and François Peyron1,2

Université Claude Bernard Lyon I, JE 1947,1 Unité d'Immunologie,3 Laboratoire de Parasitologie et Pathologie exotique,2 Hôpital de la Croix-Rousse, and Laboratoire d'Immunologie et Biologie Pulmonaire, Hôpital Louis Pradel,4 Lyon, France

Received 29 January 1998/Returned for modification 22 June 1998/Accepted 17 July 1998

To assess cell-mediated immunity to Toxoplasma gondii, we evaluated the expression of the activation antigens CD69, CD71, and CD25 on T lymphocytes by flow cytometry after specific in vitro stimulation of whole blood from 127 T. gondii-positive and 63 T. gondii-negative patients. T lymphocytes from many seropositive individuals did not express CD69 at 24 h after T. gondii antigen stimulation, but CD71 and CD25 were easily detectable on T cells from seropositive individuals 7 days after specific activation. CD25 was mainly expressed by stimulated CD4+ T cells, and its detection on total T cells was both a sensitive (98%) and a specific (97%) indicator of prior T. gondii infection. These results make flow cytometric detection of CD25 an excellent candidate for screening cell-mediated immunity to T. gondii in vitro and an interesting tool for the diagnosis of congenital infection.

* Corresponding author. Mailing address: Unité d'Immunologie, Hôpital de la Croix-Rousse, 93 Grande Rue de la Croix-Rousse, 69317 Lyon cedex 04, France. Phone: (33) 4 72 07 19 52. Fax: (33) 4 72 07 18 48. E-mail: gcozon{at}rockefeller.univ-lyon1.fr.

Clinical and Diagnostic Laboratory Immunology, November 1998, p. 745-748, Vol. 5, No. 6
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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