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Clinical and Diagnostic Laboratory Immunology, September 1998, p. 654-661, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enzyme Immunoassays for Serological Diagnosis of Bovine Brucellosis: A Trial in Latin America

D. Gall,1,* A. Colling,2 O. Marino,3 E. Moreno,4 K. Nielsen,1 B. Perez,5 and L. Samartino6

Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P91; International Atomic Energy Agency, A1400, Vienna, Austria2; Instituto Colombiano Agropecuaria, ICA-CEISA, Santafe de Bogota DC, Colombia3; Tropical Diseases Research Program, School of Veterinary Medicine, National University, Heredia, Costa Rica4; Servicios Agricolas y Ganaderos, Laboratorio Regional Osorno, Mackenna 674, Osorno, Chile5; and Instituto de Patobiologia-DPTO Bacteriologia, INTA-CICV, CC 77, 1708 Moron, Buenos Aires, Argentina6

Received 23 February 1998/Returned for modification 14 April 1998/Accepted 27 May 1998

The results of a field trial conducted in Latin America with two indirect enzyme-linked immunosorbent assays (ELISAs) and two competitive ELISAs (CELISAs) for the detection of bovine antibody to Brucella abortus are reported. One of the CELISA formats performed most accurately. The percentage of positive reactions in the CELISA relative to the selected positive rose bengal agglutination test (RBT) and complement fixation test (CFT) results was 97.47%, the percentage of negatives relative to the selected negative RBT and CFT results for unexposed cattle was 98.32%, and the percentage of negatives in cattle vaccinated with B. abortus 19 was 96.51%. The same assay format under Canadian conditions had an actual sensitivity of 100%, a specificity of 99.90% in nonvaccinates, and a specificity of 97.7% in a strain 19-vaccinated population. Overall, the CELISA performed as expected and the results were not dissimilar from the results obtained in the Canadian study. This provided further evidence that this CELISA can in many instances differentiate infected cattle from those that are vaccinated or infected with a cross-reacting organism while still giving very few false-positive or false-negative results.

* Corresponding author. Mailing address: Canadian Food Inspection Agency, Animal Diseases Research Institute, 3851 Fallowfield Road, P.O. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9. Phone: (613) 228-6698. Fax: (613) 228-6667. E-mail: gall{at}em.agr.ca.

Clinical and Diagnostic Laboratory Immunology, September 1998, p. 654-661, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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