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Clinical and Diagnostic Laboratory Immunology, September 1998, p. 617-621, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of Immunoglobulin A Antibodies to Helicobacter pylori in Serum and Gastric Juice in Relation to Mucosal Inflammation

Shunji Hayashi,1,* Toshiro Sugiyama,2 Kenji Yokota,3 Hiroshi Isogai,4 Emiko Isogai,5 Keiji Oguma,3 Masahiro Asaka,2 Nobuhiro Fujii,6 and Yoshikazu Hirai1

Department of Microbiology, Jichi Medical School, Tochigi-ken 329-0498,1 Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo 060-8638,2 Department of Bacteriology, Okayama University Medical School, Okayama 700-8558,3 Animal Experimentation Center4 and Department of Microbiology,6 Sapporo Medical University School of Medicine, Sapporo 060-8556, and Department of Hygiene, Health Sciences University of Hokkaido, Hokkaido 061-0293,5 Japan

Received 29 December 1997/Returned for modification 24 March 1998/Accepted 12 June 1998

Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori antigens were evaluated in serum and gastric juice specimens obtained from patients with gastritis or peptic ulcers by utilizing antibody capture enzyme-linked immunosorbent assays (ACELISAs). Urease alpha  subunit (UA), urease beta  subunit (UB), the 66-kDa heat shock protein (HSP), and the 25-kDa protein (25K) were used as antigens for the ACELISAs. The antibody titers of the ACELISAs reflect the ratio of H. pylori-specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. By using ACELISAs it was possible to evaluate quantitatively not only serum IgA antibodies but also gastric juice secretory IgA (S-IgA) antibodies. In both serum IgA and gastric juice S-IgA ACELISAs, the titers of antibody to HSP and 25K were remarkably correlated with the histologic grade of gastritis, whereas those to UA and UB were not strongly correlated with histologic grade. Thus, it is useful for estimating the histologic grade of gastritis to quantify serum IgA and gastric juice S-IgA antibodies to HSP and 25K.

* Corresponding author. Mailing address: Department of Microbiology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Tochigi-ken 329-0498, Japan. Phone: 81-285-58-7332. Fax: 81-285-44-1175. E-mail: shunhaya{at}jichi.ac.jp.

Clinical and Diagnostic Laboratory Immunology, September 1998, p. 617-621, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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