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Clinical and Diagnostic Laboratory Immunology, May 1998, p. 412-414, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods

Franco Guscetti,1,* Curzio Bernasconi,1 Kurt Tobler,2 Kristien Van Reeth,3 Andreas Pospischil,1 and Mathias Ackermann2

Institute of Veterinary Pathology1 and Institute of Virology,2 University of Zurich, CH-8057 Zurich, Switzerland, and Laboratory of Veterinary Virology, University of Gent, B-9822 Merelbeke, Belgium3

Received 24 September 1997/Returned for modification 5 January 1998/Accepted 3 March 1998

An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.

* Corresponding author. Mailing address: Institut fuer Veterinaerpathologie der Universitaet Zuerich, Winterthurerstr. 268, CH-8057 Zuerich, Switzerland. Phone: 41 1 6358551. Fax: 41 1 6358908. E-mail: gufo{at}vetpath.unizh.ch.

Clinical and Diagnostic Laboratory Immunology, May 1998, p. 412-414, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.