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Clinical and Diagnostic Laboratory Immunology, May 1998, p. 328-334, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Serotype Specificity of the Neutralizing-Antibody Response Induced by the Individual Surface Proteins of Rotavirus in Natural Infections of Young Children

Griselda Menchaca,1 Luis Padilla-Noriega,2,dagger Martha Méndez-Toss,2 Juan F. Contreras,1 Fernando I. Puerto,3 Hector Guiscafré,4 Felipe Mota,5 Ismael Herrera,6 Roberto Cedillo,4 Onofre Muñoz,4 Richard Ward,7 Yasutaka Hoshino,8 Susana López,2 and Carlos F. Arias2,*

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León,1 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos,2 Centro de Investigaciones Regionales "Hideyo Noguchi," Universidad Autónoma de Yucatán, Mérida, Yucatán,3 Instituto Mexicano del Seguro Social4 and Unidad de Rehidratación Oral, Hospital Infantil de México,5 Mexico City, and Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, San Luis Potosí,6 Mexico; Division of Clinical Virology, Children's Hospital Medical Center, Cincinnati, Ohio7; and National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland8

Received 10 November 1997/Returned for modification 6 January 1998/Accepted 23 February 1998

The relative contribution of the rotavirus surface proteins, VP4 and VP7, to the induction of homotypic as well as heterotypic neutralizing antibodies (NtAbs) in natural infections was studied. The NtAb titers of paired sera from 70 infants with serologically defined primary rotavirus infections were determined with a panel of rotavirus reassortants having one surface protein from a human rotavirus (serotypes G1 to G4 for VP7 and P1A and P1B for VP4) and the other surface protein from a heterologous animal rotavirus strain. A subset of 37 children were evaluated for epitope-specific antibodies to the two proteins by an epitope-blocking assay. The infants were found to seroconvert more frequently to VP4 than to VP7 by both methods, although the titers of the seroconverters were higher to VP7 than to VP4. Both proteins induced homotypic as well as heterotypic NtAbs. G1 VP7 frequently induced a response to both G1 and G3 VP7s, while G3 VP7 and P1A VP4 induced mostly homotypic responses.

* Corresponding author. Mailing address: Instituto de Biotecnología/UNAM, A.P. 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, México. Phone: (52-73) 29-1661. Fax: (52-73) 17-2388. E-mail: arias{at}ibt.unam.mx.

dagger Present address: Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.

Clinical and Diagnostic Laboratory Immunology, May 1998, p. 328-334, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.