CVI
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reyna-Bello, A.
Right arrow Articles by Zygmunt, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reyna-Bello, A.
Right arrow Articles by Zygmunt, M. S.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 259-262, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela

Armando Reyna-Bello,1,2 Axel Cloeckaert,1 Nieves Vizcaíno,1,dagger Mary I. Gonzatti,3 Pedro M. Aso,3 Gérard Dubray,1 and Michel S. Zygmunt1,*

Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France,1 and Laboratorio de Biología Experimental, Centro de Estudios Biomédicos y Veterinarios, Universidad Simón Rodríguez, Caracas 1010,2 and Laboratorio de Hemoparásitos, Departamento de Biología Celular, Universidad Simón Bolívar, Sartenejas,3 Venezuela

Received 14 July 1997/Returned for modification 2 October 1997/Accepted 8 December 1997

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of bovine anaplasmosis with purified recombinant major surface protein 5 (MSP5) of Anaplasma marginale produced in Escherichia coli. Serum antibody responses against MSP5 were detected in calves experimentally infected with A. marginale as early as 21 days postinfection and reached maximum titers at 28 days postinfection. The MSP5 ELISA performed with serum samples taken from field cattle from different regions of Venezuela showed a seroprevalence of 47%, which seems to be in accordance with the reported epidemiological status of bovine anaplasmosis in Venezuela. Positive results obtained in the MSP5 ELISA were further confirmed by immunoblotting, with the recombinant MSP5 as the antigen. Thus, these results confirmed the importance of MSP5 as a suitable antigen for the serological diagnosis of bovine anaplasmosis.

* Corresponding author. Mailing address: Laboratoire de Pathologie Infectieuse et Immunologie, INRA, 37380 Nouzilly, France. Phone: 33 2 47 42 78 67. Fax: 33 2 47 42 77 79. E-mail: zygmunt{at}tours.inra.fr.

dagger Present address: Dpto. Microbiología y Genética, Edificio Departamental, Universidad de Salamanca, Avda. Campo Charro s/n, 37007 Salamanca, Spain.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 259-262, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 1998 by the American Society for Microbiology. All rights reserved.