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Clinical and Diagnostic Laboratory Immunology, 09 1997, 624-626, Vol 4, No. 5
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus

AS Mohamed, P Becquart, H Hocini, P Metais, M Kazatchkine and L Belec
Laboratoire de Virologie, Hopital Broussais, Paris, France.

A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.

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• Gennaro, S., Raftery, N., Campbell, D., Shults, J. (2008). Cervical and Vaginal Cytokine Determinations in Pregnant Women: Methodologic Issues. Biol Res Nurs 9: 215-222 [Abstract]