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Clinical and Diagnostic Laboratory Immunology, 09 1997, 615-619, Vol 4, No. 5
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp. capripneumoniae

FR Rurangirwa, PS Shompole, AN Wambugu, SM Kihara and TC McGuire
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.

Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent- phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.