This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jenkins, M. C. Articles by Dubey, J. P. Search for Related Content PubMed PubMed Citation Articles by Jenkins, M. C. Articles by Dubey, J. P.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, 05 1997, 270-274, Vol 4, No. 3
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Serological response over time to recombinant Neospora caninum antigens in cattle after a neosporosis-induced abortion

MC Jenkins, W Wouda and JP Dubey
Parasite Biology and Epidemiology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA.

Recombinant Neospora caninum tachyzoite antigens were evaluated in an enzyme-linked immunosorbent assay (ELISA) for recognition by serum antibodies (Ab) from Neospora-infected cattle. Serum samples were obtained every 2 to 3 weeks for 8 to 15 months from 10 cows with histories of Neospora-associated abortion. Serum samples were also obtained from offspring of these animals and from a large number of cows that had aborted a fetus, due to infection by Neospora or other organisms, at various times during gestation. All 10 cows had positive ELISA Ab titers to both recombinant N. caninum tachyzoite antigens after abortion, during subsequent gestation, and after parturition. In three cows, there was a noticeable peak in Ab titers early in gestation. Calves born to Neospora-infected cows also had positive titers of Ab to the recombinant tachyzoite antigens, and these titers remained elevated for at least 4 months after birth. A portion of the serum immunoglobulin in calves may have been derived from colostrum of infected cows. A calf born from a seronegative mother had a positive ELISA titer only after being fed colostrum from a seropositive cow. However, precolostral titers in calves born from Neospora-infected cows were high at birth, suggesting that the parasite was transmitted to the fetus via the placenta and induced a humoral immune response therein. The recombinant tachyzoite antigens were also useful for corroborating clinical diagnoses of Neospora-induced abortion. A significant difference (P < 0.05) between anti-recombinant antigen Ab titers in cows that aborted due to Neospora and those in cows that aborted from other causes was found.

This article has been cited by other articles:

• Liao, M., Zhang, S., Xuan, X., Zhang, G., Huang, X., Igarashi, I., Fujisaki, K. (2005). Development of Rapid Immunochromatographic Test with Recombinant NcSAG1 for Detection of Antibodies to Neospora caninum in Cattle. CVI 12: 885-887 [Abstract] [Full Text]  
• Nishikawa, Y., Kousaka, Y., Tragoolpua, K., Xuan, X., Makala, L., Fujisaki, K., Mikami, T., Nagasawa, H. (2001). Characterization of Neospora caninum Surface Protein NcSRS2 Based on Baculovirus Expression System and Its Application for Serodiagnosis of Neospora Infection. J. Clin. Microbiol. 39: 3987-3991 [Abstract] [Full Text]  
• Jenkins, M. C., Trout, J., Murphy, C., Harp, J. A., Higgins, J., Wergin, W., Fayer, R. (1999). Cloning and Expression of a DNA Sequence Encoding a 41-Kilodalton Cryptosporidium parvum Oocyst Wall Protein. CVI 6: 912-920 [Abstract] [Full Text]  
• Wouda, W., Brinkhof, J., van Maanen, C., de Gee, A. L. W., Moen, A. R. (1998). Serodiagnosis of Neosporosis in Individual Cows and Dairy Herds: A Comparative Study of Three Enzyme-Linked Immunosorbent Assays. CVI 5: 711-716 [Abstract] [Full Text]