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Clinical and Diagnostic Laboratory Immunology, Nov 1996, 779-781, Vol 3, No. 6
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

A new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant

EM Mascini, MA Hazenberg, LA Verhage, SE Holm, J Verhoef and H van Dijk
Eijkman-Winkler Institute for Microbiology, Utrecht University Hospital, The Netherlands.

An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (> or = 99%), and time.

This article has been cited by other articles:

• Mascini, E. M., Jansze, M., Verhoef, J., van Dijk, H. (1999). Relative Avidities of Human Immunoglobulin G Antibodies for Streptococcal Pyrogenic Exotoxins A and B. CVI 6: 977-980 [Abstract] [Full Text]  
• Mascini, E. M., Jansze, M., Schouls, L. M., Fluit, A. C., Verhoef, J., van Dijk, H. (1999). Invasive and Noninvasive Group A Streptococcal Isolates with Different speA Alleles in The Netherlands: Genetic Relatedness and Production of Pyrogenic Exotoxins A and B. J. Clin. Microbiol. 37: 3469-3474 [Abstract] [Full Text]