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Clinical and Diagnostic Laboratory Immunology, 09 1996, 614-615, Vol 3, No. 5
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

False-positive serologic test resulting from a probable yeast infection in a chimpanzee

T Heller, S Yin, SA Tsarev, RH Purcell and SU Emerson
Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0740, USA. Theller@atlas.niaid.nih.gov

Sera used to identify putative hepatitis E viral proteins expressed in Pischia pastoris produced a false-positive reaction because of antibodies to a yeast protein. This report illustrates a potential problem when serological reagents are used in combination with recombinant proteins expressed in yeast.




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 1996 by the American Society for Microbiology. All rights reserved.