This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Harning, D. Articles by Petersen, E. Search for Related Content PubMed PubMed Citation Articles by Harning, D. Articles by Petersen, E.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, 05 1996, 355-357, Vol 3, No. 3
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Recombinant Toxoplasma gondii surface antigen 1 (P30) expressed in Escherichia coli is recognized by human Toxoplasma-specific immunoglobulin M (IgM) and IgG antibodies

D Harning, J Spenter, A Metsis, J Vuust and E Petersen
Laboratory of Molecular Biology, Statens Seruminstitut, Copenhagen, Denmark.

The immunodominant surface antigen of Toxoplasma gondii, surface antigen 1 (SAG1), was expressed in Escherichia coli as a fusion protein containing a majority of the SAG1 protein supplied with six histidyl residues in the N-terminal end. The recombinant protein was purified on a Ni-chelate column and then on a fast-performance liquid chromatography column and was in a nonreduced condition. It was recognized by T. gondii-specific human immunoglobulin G (IgG) and IgM antibodies as well as by a mouse monoclonal antibody (S13) recognizing only nonreduced native SAG1. Antibodies induced in mice by the recombinant SAG1 recognized native SAG1 from the T. gondii RH isolate in culture. Recombinant SAG1 is suitable for use in diagnostic systems for detecting anti-SAG1-specific IgG and IgM antibodies.

This article has been cited by other articles:

• Buffolano, W., Beghetto, E., Del Pezzo, M., Spadoni, A., Di Cristina, M., Petersen, E., Gargano, N. (2005). Use of Recombinant Antigens for Early Postnatal Diagnosis of Congenital Toxoplasmosis. J. Clin. Microbiol. 43: 5916-5924 [Abstract] [Full Text]  
• Pfrepper, K.-I., Enders, G., Gohl, M., Krczal, D., Hlobil, H., Wassenberg, D., Soutschek, E. (2005). Seroreactivity to and Avidity for Recombinant Antigens in Toxoplasmosis. CVI 12: 977-982 [Abstract] [Full Text]  
• Ferguson, D. J. P., Henriquez, F. L., Kirisits, M. J., Muench, S. P., Prigge, S. T., Rice, D. W., Roberts, C. W., McLeod, R. L. (2005). Maternal Inheritance and Stage-Specific Variation of the Apicoplast in Toxoplasma gondii during Development in the Intermediate and Definitive Host. Eukaryot Cell 4: 814-826 [Abstract] [Full Text]  
• Sorensen, T., Spenter, J., Jaliashvili, I., Christiansen, M., Norgaard-Pedersen, B., Petersen, E. (2002). Automated Time-resolved Immunofluorometric Assay for Toxoplasma gondii-specific IgM and IgA Antibodies: Study of More Than 130 000 Filter-Paper Blood-Spot Samples from Newborns. Clin. Chem. 48: 1981-1986 [Abstract] [Full Text]  
• Suzuki, Y., Ramirez, R., Press, C., Li, S., Parmley, S., Thulliez, P., Remington, J. S. (2000). Detection of Immunoglobulin M Antibodies to P35 Antigen of Toxoplasma gondii for Serodiagnosis of Recently Acquired Infection in Pregnant Women. J. Clin. Microbiol. 38: 3967-3970 [Abstract] [Full Text]  
• Aubert, D., Maine, G. T., Villena, I., Hunt, J. C., Howard, L., Sheu, M., Brojanac, S., Chovan, L. E., Nowlan, S. F., Pinon, J. M. (2000). Recombinant Antigens To Detect Toxoplasma gondii-Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay. J. Clin. Microbiol. 38: 1144-1150 [Abstract] [Full Text]  
• Nielsen, H. V., Lauemoller, S. L., Christiansen, L., Buus, S., Fomsgaard, A., Petersen, E. (1999). Complete Protection against Lethal Toxoplasma gondii Infection in Mice Immunized with a Plasmid Encoding the SAG1 Gene. Infect. Immun. 67: 6358-6363 [Abstract] [Full Text]  
• Jacobs, D., Vercammen, M., Saman, E. (1999). Evaluation of Recombinant Dense Granule Antigen 7 (GRA7) of Toxoplasma gondii for Detection of Immunoglobulin G Antibodies and Analysis of a Major Antigenic Domain. CVI 6: 24-29 [Abstract] [Full Text]  
• Martin, V., Arcavi, M., Santillan, G., Amendoeira, M. R. R., De Souza Neves, E., Griemberg, G., Guarnera, E., Garberi, J. C., Angel, S. O. (1998). Detection of Human Toxoplasma-Specific Immunoglobulins A, M, and G with a Recombinant Toxoplasma gondii Rop2 Protein. CVI 5: 627-631 [Abstract] [Full Text]  
• Letscher-Bru, V., Villard, O., Risse, B., Zauke, M., Klein, J.-P., Kien, T. T. (1998). Protective Effect of Vaccination with a Combination of Recombinant Surface Antigen 1 and Interleukin-12 against Toxoplasmosis in Mice. Infect. Immun. 66: 4503-4506 [Abstract] [Full Text]