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Clinical and Diagnostic Laboratory Immunology, May 1996, 351-354, Vol 3, No. 3
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

A monoclonal antibody reactive with a common epitope of Moraxella (Branhamella) catarrhalis lipopolysaccharides

K Oishi, H Tanaka, F Sonoda, S Borann, K Ahmed, Y Utsunomiya, K Watanabe, T Nagatake, M Vaneechoutte, G Verschraegen and K Matsumoto
Department of Internal Medicine, Nagasaki University, Japan.

A hybrid cell line producing a monoclonal antibody (MAb) against Moraxella (Branhamella) catarrhalis lipopolysaccharide (LPS) was established. The specificity of the MAb 1B12 to purified rough LPSs from six strains of M. catarrhalis was ascertained by enzyme-linked immunosorbent assay (ELISA), competitive-inhibition ELISA, and immunoblotting. MAb 1B12 bound to live bacterial cells and culture supernatants from a total of 34 strains of M. catarrhalis, including 12 strains with different LPS serotypes. No cross-reactions with smooth and rough LPSs from selected enterobacterial and nonenterobacterial strains, with other respiratory pathogens, or with Neisseria species were observed. These data suggest that MAb 1B12 recognizes a common epitope of M. catarrhalis LPS which differs from serotype determinants.

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